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利用微流控技术操控单细胞或微粒是近年来的研究热点之一，但对目标细胞或粒子的鉴定识别及选择性回收仍然是其研究难点。介绍了一种新的微粒操控方法，将微流控技术与用于3D打印的数字光处理(DLP)投影面曝光技术结合，可以在单片上完成单细胞或微粒的捕获及定点固定。制作了捕获阵列微流控芯片，芯片进行细胞或微粒捕获后，通过搭建的光学运动平台对捕获结果进行识别，通过图像算法自动生成投影图像，并导入生物相容性可光固化聚乙二醇双丙烯酸酯(PEGDA)水凝胶，采用DLP投影系统在目标处投影使PEGDA固化，完成对单细胞或微粒的固定，最后利用缓冲液将未投影的细胞或微粒释放。通过微粒的捕获和固定实验，初步验证了该方法进行大规模微粒或细胞捕获和分选操作的可行性，为单细胞或微粒研究应用提供了一个新的技术手段。
Abstract：The use of microfluidics technology to manipulate single cell or particle is one of the hot issues in recent years, but the identification and selective recovery of target cells or particles in the research are still difficult. A new method of particle manipulation was introduced. The method combines microfluidics technology with digital light processing(DLP) projection surface exposure technology for 3 D printing, and can accomplish single cell or particle capture and fixed-point immobilization on a single chip. A trap array microfluidic chip was fabricated. After cells or particles captured by the chip, the captured result was identified by the built-up optical motion platform, and the projection image was automatically generated by the image algorithm. The biocompatible light-curable polyethylene glycol diacrylate(PEGDA) hydrogel was imported and then solidified by the DLP projection system, which can immobilize single cell or particle. At last the unprojected cells or particles were released by buffer solution. Through particle capture and immobilization experiment, the feasibility of large-scale particles or cells capture and sorting was preliminarily verified, providing a new technical method for research and application of single cell or particle.
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基本信息:
DOI：10.13250/j.cnki.wndz.2022.06.011
中图分类号:R446;TN492
引用信息:
[1]龙妍婷,张楚,陈晓霞,等.基于微流控和投影面曝光的微粒操控技术[J].微纳电子技术,2022,59(06):570-577.DOI:10.13250/j.cnki.wndz.2022.06.011.
基金信息:
上海市科委基础重大研发计划资助项目(17JC1401001)
2022-03-07
2022-03-07
2022-03-07