微纳电子技术

基于T矩阵模型,推导出单模光纤光镊对细胞的驱动力公式。以COMSOL Multiphysics5.3为仿真软件,首先模拟出单模光纤的磁场模和电场模分布,得出在纤芯处场强最大,激光聚焦于此,然后又模拟出同一功率和波长条件下的光纤光镊对不同直径微粒的驱动作用,模拟结果表明不同直径的微粒沿着光轴移动的距离不等。提出了用于细胞驱动的光纤芯片的制作方法,并在波长为980 nm、功率为300 mW激光条件下对聚苯乙烯微球和酵母菌细胞的混合液进行了驱动实验。该实验结果表明,在同种条件下,聚焦激光可以通过单模光纤驱动不同直径的细胞沿光轴方向移动,且移动距离不相等,为下一步细胞分选提供了理论和实验基础。

Based on the T-matrix model,the driving force formula of single-mode optical fiber tweezers for cells was derived.Using COMSOL Multiphysics 5.3 as the simulation software,the magnetic field mode distribution and electric field mode distribution of the single-mode optical fiber were simulated firstly.The results show that the field intensity is the highest at the fiber core,so the laser is focused on it.Then the driving effect of fiber optical tweezers on particles with different diameters under the same power and wavelength was simulated.The result shows that the moving distances of microparticles with different diameters along the optical axis are not equal.A method of processing fiber chips for driving cells was proposed,and the drive experiment of the mixed solution of polystyrene microspheres and yeast cells was carried out under the laser condition of 980 nm wavelength and 300 mW power.The experimental results show that under the same condition,the focused laser can drive the cells with different diameters to move along the optical axis direction through the single-mode fiber,and the moving distance was not equal,providing the theoretical and experimental basis for the next cell sorting.